Review



immun blot low fluorescence pvdf membrane  (Bio-Rad)


Bioz Verified Symbol Bio-Rad is a verified supplier
Bioz Manufacturer Symbol Bio-Rad manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96

    Structured Review

    Bio-Rad immun blot low fluorescence pvdf membrane
    Immun Blot Low Fluorescence Pvdf Membrane, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 21611 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/low+fluorescence+pvdf+membrane/Immun-Blot+PVDF+Membrane/pmc13175989-63-25-30
    Average 96 stars, based on 21611 article reviews
    immun blot low fluorescence pvdf membrane - by Bioz Stars, 2026-09
    96/100 stars

    Images

    Related Articles

    Membrane:

    Article Title: Nucleotide-resolution mapping of regulatory elements via allelic readout of tiled base editing.
    Article Snippet: Proteins were denatured using XT Sample Buffer (BioRad, #1610791) for 5 min at 95 °C and separated in 4-12% Criterion XT Bis-Tris Protein Gels (BioRad, #3450125). .. Proteins were then transferred to a low-fluorescence PVDF membrane (BioRad, #1704275) using the High MW program of the Trans-Blot Turbo Transfer System (BioRad, #1704150). .. The membranes were blocked for 1 h, AR TI CL E IN P RE SS ARTICLE IN PRESS incubated overnight at 4 °C in primary antibody (1: 1,000 MYB – Proteintech, 17800-1-AP; 1: 20,000 b-Actin – Santa Cruz, sc-47778) and at RT for 2 h in secondary antibody (1: 10,000 antirabbit – Cell Signaling, #7074) in EveryBlot blocking buffer (BioRad, #12010020).

    Article Title: A toolbox for ablating excitatory and inhibitory synapses
    Article Snippet: 30 μg of cell lysate was denatured in Laemmli Buffer (Bio-Rad, cat. # 1610747) with 10% 2-mercaptoethanol and ran on precast Any kD SDS–PAGE protein gels (Bio-Rad, cat. # 4569034). .. Proteins were then transferred onto low-fluorescence PVDF membrane (Bio-Rad, cat. # 1620264) and probed with chicken anti-myc (1:3000, Novus Biologicals NB600-334) and mouse anti- α-tubulin (1:5000, Sigma T6199). .. Secondary antibodies were goat anti-chicken Alexa Fluor 680 (1:1000, Abcam, cat. # ab175779) and goat anti-mouse Alexa Fluor 750 (1:1000, Thermo Fisher Scientific, cat. # A-21037).

    Article Title: Nucleotide-resolution mapping of regulatory elements via allelic readout of tiled base editing
    Article Snippet: Proteins were denatured using XT Sample Buffer (BioRad, #1610791) for 5 min at 95 °C and separated in 4–12% Criterion XT Bis-Tris Protein Gels (BioRad, #3450125). .. Proteins were then transferred to a low-fluorescence PVDF membrane (BioRad, #1704275) using the High MW program of the Trans-Blot Turbo Transfer System (BioRad, #1704150). .. The membranes were blocked for 1 h, incubated overnight at 4 °C in primary antibody (1:1000 MYB – Proteintech, 17800-1-AP; 1: 20,000 b-Actin – Santa Cruz, sc-47778) and at RT for 2 h in secondary antibody (1: 10,000 anti-rabbit – Cell Signaling, #7074) in EveryBlot blocking buffer (BioRad, #12010020).

    Article Title: Network-based insights into miRNA regulation of β-cell insulin secretion in type 2 diabetes
    Article Snippet: .. Low Fluorescence PVDF Membrane , Bio-Rad , Cat#1620260. .. Clarity Western ECL Substrate , Bio-Rad , Cat#1705061.

    Article Title: Fluorescence‐Based Multiplex Western Blot to Simultaneously Detect the Insulin‐Like Growth Factor‐1 (IGF‐1) Isoforms
    Article Snippet: .. The samples were separated by SDS‐PAGE (15% acrylamide) in the conventional SDS‐glycine buffer system and transferred to 0.2 μm nitrocellulose membrane (catalog #1620112; Bio‐Rad), or low fluorescence PVDF membrane (catalog #1620264; Bio‐Rad). .. After transfer, the membranes were blocked with filtered 5% BSA in Tris‐buffered saline (TBS) or with EveryBlot Blocking Buffer (catalog #12010020; Bio‐Rad) at room temperature while shaking for 1 h. After blocking, membranes were incubated overnight at 4°C with a single or a mix of the following primary antibodies resuspended in 5% BSA in TBS‐T (TBS plus 0.05% Tween 20): the rabbit polyclonal anti‐human IGF‐1 antibody which recognizes the core epitope (1:2000 corresponding to 0.125 μg/mL; catalog #500P11; PeproTech) and the goat IGF‐1 polyclonal Ab against E‐peptide domains (1:2000 corresponding to 0.25 μg/mL; catalog #PA5‐19382; Invitrogen).

    Article Title: Control of Golgi- V-ATPase through Sac1-dependent co-regulation of PI(4)P and cholesterol
    Article Snippet: Pageruler Plus prestained protein ladder (Thermo Fisher, 26619) was used. .. Gels were activated with the ChemiDoc MD Imaging System (Bio-Rad) and transferred to a 0.45 μm Low Fluorescence PVDF membrane (Bio-Rad, 1704274). .. Membranes were blocked for 45 min at RT with 5% skim milk (Valio) in 0.1% Tween-20 (Fisher Scientific, BP337) in TBS (TBS-t), and subsequently incubated with the primary antibody overnight at 4 °C.

    Article Title: Oocyte polarity is established independently of the Balbiani body
    Article Snippet: Oocyte samples were suspended in NP-40 lysis buffer with protease inhibitor (Rosch Cat# 04693124001) and homogenized with a micro pestle on ice. .. Samples were denatured with Laemmli sample buffer (BioRad Cat# 1610737) at 95 °C for 5 min before loading on a 4–15% Mini-PROTEAN TGX Precast Gel (BioRad Cat# 4561086) and blotted on a low-fluorescence PVDF membrane (BioRad Cat# 1620261) using XCell II Blot Module (Invitrogen, EI9051). .. For western blot normalization, total protein was stained with RevertTM 700 Total Protein Stain (LI-COR Cat# 926-11010) and imaged on a LI-COR ODYSSEY M. Following total protein staining, the membrane was cut in half for detecting Buc and Actin as a positive control.

    Article Title: Fluorescence-Based Multiplex Western Blot to Simultaneously Detect the Insulin-Like Growth Factor-1 (IGF-1) Isoforms.
    Article Snippet: .. The samples were separated by SDS-PAGE (15% acrylamide) in the conventional SDS-glycine buffer system and transferred to 0.2 μm nitrocellulose membrane (catalog #1620112; Bio-Rad), or low fluorescence PVDF membrane (catalog #1620264; Bio-Rad). .. After transfer, the membranes were blocked with filtered 5% BSA in Tris-buffered saline (TBS) or with EveryBlot Blocking Buffer (catalog #12010020; Bio-Rad) at room temperature while shaking for 1 h. After blocking, membranes were incubated overnight at 4◦C with a single or a mix of the following primary antibodies resuspended in 5% BSA in TBS-T (TBS plus 0.05% Tween 20): the rabbit polyclonal antihuman IGF-1 antibody which recognizes the core epitope (1:2000 corresponding to 0.125 μg/mL; catalog #500P11; PeproTech) and the goat IGF-1 polyclonal Ab against E-peptide domains (1:2000 corresponding to 0.25 μg/mL; catalog #PA5-19382; Invitrogen).

    Fluorescence:

    Article Title: Network-based insights into miRNA regulation of β-cell insulin secretion in type 2 diabetes
    Article Snippet: .. Low Fluorescence PVDF Membrane , Bio-Rad , Cat#1620260. .. Clarity Western ECL Substrate , Bio-Rad , Cat#1705061.

    Article Title: Fluorescence‐Based Multiplex Western Blot to Simultaneously Detect the Insulin‐Like Growth Factor‐1 (IGF‐1) Isoforms
    Article Snippet: .. The samples were separated by SDS‐PAGE (15% acrylamide) in the conventional SDS‐glycine buffer system and transferred to 0.2 μm nitrocellulose membrane (catalog #1620112; Bio‐Rad), or low fluorescence PVDF membrane (catalog #1620264; Bio‐Rad). .. After transfer, the membranes were blocked with filtered 5% BSA in Tris‐buffered saline (TBS) or with EveryBlot Blocking Buffer (catalog #12010020; Bio‐Rad) at room temperature while shaking for 1 h. After blocking, membranes were incubated overnight at 4°C with a single or a mix of the following primary antibodies resuspended in 5% BSA in TBS‐T (TBS plus 0.05% Tween 20): the rabbit polyclonal anti‐human IGF‐1 antibody which recognizes the core epitope (1:2000 corresponding to 0.125 μg/mL; catalog #500P11; PeproTech) and the goat IGF‐1 polyclonal Ab against E‐peptide domains (1:2000 corresponding to 0.25 μg/mL; catalog #PA5‐19382; Invitrogen).

    Article Title: Control of Golgi- V-ATPase through Sac1-dependent co-regulation of PI(4)P and cholesterol
    Article Snippet: Pageruler Plus prestained protein ladder (Thermo Fisher, 26619) was used. .. Gels were activated with the ChemiDoc MD Imaging System (Bio-Rad) and transferred to a 0.45 μm Low Fluorescence PVDF membrane (Bio-Rad, 1704274). .. Membranes were blocked for 45 min at RT with 5% skim milk (Valio) in 0.1% Tween-20 (Fisher Scientific, BP337) in TBS (TBS-t), and subsequently incubated with the primary antibody overnight at 4 °C.

    Article Title: Fluorescence-Based Multiplex Western Blot to Simultaneously Detect the Insulin-Like Growth Factor-1 (IGF-1) Isoforms.
    Article Snippet: .. The samples were separated by SDS-PAGE (15% acrylamide) in the conventional SDS-glycine buffer system and transferred to 0.2 μm nitrocellulose membrane (catalog #1620112; Bio-Rad), or low fluorescence PVDF membrane (catalog #1620264; Bio-Rad). .. After transfer, the membranes were blocked with filtered 5% BSA in Tris-buffered saline (TBS) or with EveryBlot Blocking Buffer (catalog #12010020; Bio-Rad) at room temperature while shaking for 1 h. After blocking, membranes were incubated overnight at 4◦C with a single or a mix of the following primary antibodies resuspended in 5% BSA in TBS-T (TBS plus 0.05% Tween 20): the rabbit polyclonal antihuman IGF-1 antibody which recognizes the core epitope (1:2000 corresponding to 0.125 μg/mL; catalog #500P11; PeproTech) and the goat IGF-1 polyclonal Ab against E-peptide domains (1:2000 corresponding to 0.25 μg/mL; catalog #PA5-19382; Invitrogen).

    Imaging:

    Article Title: Control of Golgi- V-ATPase through Sac1-dependent co-regulation of PI(4)P and cholesterol
    Article Snippet: Pageruler Plus prestained protein ladder (Thermo Fisher, 26619) was used. .. Gels were activated with the ChemiDoc MD Imaging System (Bio-Rad) and transferred to a 0.45 μm Low Fluorescence PVDF membrane (Bio-Rad, 1704274). .. Membranes were blocked for 45 min at RT with 5% skim milk (Valio) in 0.1% Tween-20 (Fisher Scientific, BP337) in TBS (TBS-t), and subsequently incubated with the primary antibody overnight at 4 °C.

    SDS Page:

    Article Title: Fluorescence-Based Multiplex Western Blot to Simultaneously Detect the Insulin-Like Growth Factor-1 (IGF-1) Isoforms.
    Article Snippet: .. The samples were separated by SDS-PAGE (15% acrylamide) in the conventional SDS-glycine buffer system and transferred to 0.2 μm nitrocellulose membrane (catalog #1620112; Bio-Rad), or low fluorescence PVDF membrane (catalog #1620264; Bio-Rad). .. After transfer, the membranes were blocked with filtered 5% BSA in Tris-buffered saline (TBS) or with EveryBlot Blocking Buffer (catalog #12010020; Bio-Rad) at room temperature while shaking for 1 h. After blocking, membranes were incubated overnight at 4◦C with a single or a mix of the following primary antibodies resuspended in 5% BSA in TBS-T (TBS plus 0.05% Tween 20): the rabbit polyclonal antihuman IGF-1 antibody which recognizes the core epitope (1:2000 corresponding to 0.125 μg/mL; catalog #500P11; PeproTech) and the goat IGF-1 polyclonal Ab against E-peptide domains (1:2000 corresponding to 0.25 μg/mL; catalog #PA5-19382; Invitrogen).



    Similar Products

    96
    Bio-Rad immun blot low fluorescence pvdf membrane
    Immun Blot Low Fluorescence Pvdf Membrane, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/low+fluorescence+pvdf+membrane/Immun-Blot+PVDF+Membrane/pmc13175989-63-25-30
    Average 96 stars, based on 1 article reviews
    immun blot low fluorescence pvdf membrane - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    96
    Bio-Rad fluorescence pvdf membrane
    Fluorescence Pvdf Membrane, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/low+fluorescence+pvdf+membrane/Immun-Blot+Low+Fluorescence+PVDF%2FFilter+Paper+Sets/pm41991671-233-25-29
    Average 96 stars, based on 1 article reviews
    fluorescence pvdf membrane - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    96
    Bio-Rad low auto fluorescence pvdf membranes
    Low Auto Fluorescence Pvdf Membranes, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/low+fluorescence+pvdf+membrane/Immun-Blot+Low+Fluorescence+PVDF%2FFilter+Paper+Sets/pm41991007-213-14-18
    Average 96 stars, based on 1 article reviews
    low auto fluorescence pvdf membranes - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    96
    Bio-Rad low fluorescence pvdf membranes
    Low Fluorescence Pvdf Membranes, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/low+fluorescence+pvdf+membrane/Trans-Blot+Turbo+Mini+PVDF+Transfer+Packs/pmc13140717-78-14-19
    Average 96 stars, based on 1 article reviews
    low fluorescence pvdf membranes - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    96
    Bio-Rad lf pvdf membrane
    Lf Pvdf Membrane, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/low+fluorescence+pvdf+membrane/Immun-Blot+Low+Fluorescence+PVDF%2FFilter+Paper+Sets/bio_rxiv__64898__2026__03__26__714539-251-24-26
    Average 96 stars, based on 1 article reviews
    lf pvdf membrane - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    96
    Bio-Rad pvdf membranes
    Pvdf Membranes, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/low+fluorescence+pvdf+membrane/Immun-Blot+Low+Fluorescence+PVDF%2FFilter+Paper+Sets/pm41825779-134-10-12
    Average 96 stars, based on 1 article reviews
    pvdf membranes - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    Image Search Results